The effect of post-induction nutrient feeding strategies on the production of bioadhesive protein using an IPTG inducible expression system in Escherichia coli was investigated. Cells were cultured in an exponen- tial fed-batch mode to the OD 600 of ca. 100 (48 gDCW/L) prior to induction. Six differ
Effects of post-induction feed strategies on secretory production of recombinant streptokinase in Escherichia coli
β Scribed by Subramanian Ramalingam; Pennathur Gautam; Krishna Jyoti Mukherjee; Guhan Jayaraman
- Publisher
- Elsevier Science
- Year
- 2007
- Tongue
- English
- Weight
- 512 KB
- Volume
- 33
- Category
- Article
- ISSN
- 1369-703X
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Two Escherichia coli strains, widely used for the production of various recombinant proteins, were compared for their pre-induction growth and acetate accumulation patterns. The strains studied were β¬, coliBL21 (ADES), transformed with a plasmid encoding Pseudomonas exotoxin A, and an β¬. coli K12 de