𝔖 Bobbio Scriptorium
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Effects of diapause on lethalYellow (Ay/Ay) mouse embryos

✍ Scribed by Papaioannou, Virginia E. ;Gardner, Richard L.

Book ID
102890734
Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
702 KB
Volume
263
Category
Article
ISSN
0022-104X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

One of the problems in studying early acting recessive lethal genes is recognizing the homozygotes prior to their demise. Molecular probes can assist in this task, but their use generally requires removal of cells and consequent damage to the embryo, which may compromise its subsequent development. The present study was undertaken in an attempt to distinguish intact, living, lethal yellow (A^y^/A^y^) embryos from A^y^/a^e^ and a^e^/a^e^ littermates before implantation by placing them in implantation delay or diapause. After 2 days in the reproductive tract of prepubertal females, the great majority of presumptive lethal A^y^/A^y^ embryos had failed to hatch from the zona pellucida and they exhibited a marked deficiency of cells relative to controls, particularly in the inner cell mass. This argues against a stage‐specific role for the gene in implantation. Β© 1992 Wiley‐Liss, Inc.

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