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Effects of caffeine, cumulus cell removal and aging on polyspermy and embryo development on in vitro matured and fertilized ovine oocytes

โœ Scribed by W.E. Maalouf; J.-H. Lee; K.H.S. Campbell


Book ID
116911870
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
492 KB
Volume
71
Category
Article
ISSN
0093-691X

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Cumulus cell function during bovine oocy
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## Abstract Several contemporary micromanipulation techniques, such as sperm microinjection, nuclear transfer, and gene transfer by pronuclear injection, require removal of cumulus cells from oocytes or zygotes at various stages. In humans, the cumulus cells are often removed after 15โ€“18 hr of sper