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Influence of sperm:oocyte ratio during in vitro fertilization of in vitro matured cumulus-intact pig oocytes on fertilization parameters and embryo development

โœ Scribed by Maria A. Gil; Maria Ruiz; Cristina Cuello; Juan M. Vazquez; Jordi Roca; Emilio A. Martinez


Book ID
117271054
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
163 KB
Volume
61
Category
Article
ISSN
0093-691X

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## Abstract Several contemporary micromanipulation techniques, such as sperm microinjection, nuclear transfer, and gene transfer by pronuclear injection, require removal of cumulus cells from oocytes or zygotes at various stages. In humans, the cumulus cells are often removed after 15โ€“18 hr of sper