Effect of the Removal of Cumulus Cells on the Nuclear Maturation, Fertilization and Development of Porcine Oocytes
β Scribed by P Wongsrikeao; Y Kaneshige; R Ooki; M Taniguchi; B Agung; M Nii; T Otoi
- Book ID
- 108921601
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 89 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0936-6768
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The influence of small doses of ethanol or dimethylsulphoxide (DMSO) on in vitro maturation (IVM) of bovine cumulus-oocyte complexes (COC) was examined, either after spontanous maturation or after inhibition of meiosis with 6-dimethylaminopurine (6-DMAP) or 3-isobutyl-1-methylxanthine (IBMX). Subseq
## Abstract Follicle cell numbers, steroidogenesis, and culture conditions were manipulated in a series of experiments designed to identify their effect during in vitro maturation of pig oocytes upon meiotic cycle progression, sperm penetration and male pronuclear formation. In Experiment 1 a stati