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Effects of AC-Electrolysis on the Enzymatic Activity of Glucose Oxidase

✍ Scribed by Malika Ammam; Jan Fransaer

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
533 KB
Volume
23
Category
Article
ISSN
1040-0397

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✦ Synopsis

Abstract

The change in the activity of glucose oxidase subjected to an asymmetrical alternating current (AC) electric field is investigated via horseradish peroxidase (HRP)‐coupled bioassay. The effect of the amplitude, frequency and enzyme concentration have been shown to affect the enzyme activity towards glucose oxidation. The decrease in the enzyme activity is directly related to the change in pH and temperature of the GOx solution during AC electrolysis. The enzyme activity reduces with increasing amplitude, enzyme concentration and decreasing frequency. Results from UV‐vis, FT‐IR and UV CD spectroscopy showed that the AC treated GOx samples undergo structural modifications.

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