Effect of water activity on thermolysin-catalyzed peptide synthesis in organic solvents
β Scribed by Kyung-A Hwang; Sun Bok Lee; Kyung Hee Lee
- Publisher
- Springer Netherlands
- Year
- 1995
- Tongue
- English
- Weight
- 483 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0141-5492
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π SIMILAR VOLUMES
We have studied a thermolysin-catalyzed solidto-solid dipeptide synthesis using equimolar amounts of Z-Gln-OH and H-Leu-NH 2 as model substrates. The high substrate concentrations make this an effective alternative to enzymatic peptide synthesis in organic solvents. Water content was varied in the r
Free Staphylococcus auseus V8 proteinase was applied to coupling reactions of Z-Glu-OMe, Z-Glu-OBzl, Z-Ala-Glu-OMe and Z-Ala-Ala-Glu-OMe with various amino acid amides and peptides in acetonitrile containing 5 vol. % of water. No synthesis of Z-Asp-Leu-NH, was achieved under the same conditions. The