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Effect of Vanadate on Different Forms of Coomassie Brilliant Blue and Protein Assay

✍ Scribed by Mohammed Momin Shareef; K.Taranath Shetty

Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
150 KB
Volume
258
Category
Article
ISSN
0003-2697

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✦ Synopsis

enough for the characterization of cationic lipid-mediated gene transfer in various adherent cells. If an increase of sensitivity is required, either buffers other than HBSS and Tris-HCl can be used or a higher incubation temperature can be employed. Nevertheless, we found this assay highly suitable for our purposes allowing fast, easy, and inexpensive determination of cell viability and reporter gene expression after lipofection, and thus a characterization of cationic liposomes regarding their transfection properties.

Acknowledgments. The authors are grateful to the technical assistance of Ba Β¨rbel Pohl and Jana Richter. This work was supported in part by a grant from the Deutsche Forschungsgesellschaft (SFB 507).

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An automatic method for the protein assay using Coomassie Brilliant Blue G-250 was developed and applied to the assay of urinary proteins. In developing the automatic system, the adhesion of protein-bound dye to the walls of the flow cell and tubes was found to be the most troublesome problem, by wh