EFFECT OF SUBSTRATE SIZE ON THE ACTIVITY OF TOMATO POLYGALACTURONASE
β Scribed by R. PRESSEY; J. K. AVANTS
- Book ID
- 108799543
- Publisher
- Institute of Food Technologists
- Year
- 1971
- Tongue
- English
- Weight
- 489 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0022-1147
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π SIMILAR VOLUMES
## Abstract Immunoinhibition assays are hypothesized to work by antibodies blocking substrate access to enzyme active sites. To test this hypothesis, the inhibition of amylase isoenzymes by monoclonal and polyclonal antisera was assessed using substrates of varying sizes: chromogenic sustrates 3, 5
Juices made from tomatoes containing antisense genes for the enzymes polygalacturonase (PG(as)) and pectin esterase isoform 2 (PE(as)) were compared to those made from normal fruit. The juice from the PG(as) fruit was thicker than that from the normal fruit. This increased viscosity was shown to dev