Effect of sonic disruption of erythrocyte stromata on the glucose, phlorizin and phloretin inhibition of the stromal adenosinetriphosphatase

✍ Scribed by Ewers, A. C. ;Haskell, C. M. ;Fineberg, R. A.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1963
• Tongue: English
• Weight: 498 KB
• Volume: 61
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030610209

✦ Synopsis

Herbert ('56) studied in some detail an enzyme of human red blood cell membranes which could liberate inorganic phosphate from adenosine triphosph ate (ATP). Post, et al. ('60) collected evidence favoring the hypothesis that this enzyme is involved in sodium and potassium transport. The ATPase was studied further in human, rabbit, and bovine erythrocyte stromata by Laris, et al. ('60 and '62), who have shown a correlation between inhibition of glucose transport and inhibition of ATPase activity by aldoses, phloretin, and phlorizin. Since the hydrolysis of ATP does not seem to be essential for sugar penetration, it was suggested that the enzyme and the mechanism involved in sugar penetration had structural features in common.

This inhibition of ATPase might result from a fortuitous topographic relationship with the binding sites for the inhibitors of glucose permeability such that the inhibitors interfere with the enzyme. If it were possible to separate the sites, then the inhibition of the ATPase should be abolished. The present experiments represent an attempt to accomplish this s e p aration by fragmenting the erythrocyte stroma with sonic oscillation. Preliminary experiments showed that the activity of the enzyme remained at a useful, although diminished, level after sonic fragmentation of the membrane.