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EFFECT OF SODIUM LAURYL SULFATE IN SOLUBILIZING PROTEIN FOR TRYPTOPHAN DETERMINATION

โœ Scribed by EDMAR CLEMENTE; FABIO DE BORJA PORTELA

Book ID
111336809
Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
309 KB
Volume
12
Category
Article
ISSN
0145-8884

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A simple method for determination of proteins, initially solubilized in Tris buffer containing sodium dodecyl sulfate (SDS), mercaptoethanol, and sucrose, is described. This method is based on protein and Coomassie brilliant blue G-250 binding but it involves the removal of excess SDS by precipitati