Effect of SecB Chaperone on Production of Periplasmic Penicillin Acylase in Escherichia coli
โ Scribed by C. Perry Chou; Jen-Hung Tseng; Bao-Yuan Kuo; Ko-Ming Lai; Ming-I Lin; Hua-Kuo Lin
- Book ID
- 109388225
- Publisher
- American Institute of Chemical Engineers
- Year
- 1999
- Tongue
- English
- Weight
- 138 KB
- Volume
- 15
- Category
- Article
- ISSN
- 8756-7938
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## Abstract The extracellular production of penicillin acylase (PAC) in genetically engineered __Escherichia coli__ by coexpression of the __brp__ gene encoding bacteriocin release protein (BRP) and the __pac__ gene was demonstrated. Cell physiology was affected while PAC was released into the medi
The stabilisation of Escherichia coli penicillin G acylase (PGA) by dextran polymers (of molecular weight 11.5, 37.7 and 71 kDa) was studied. The inactivation of both the native and dextrancontaining enzyme preparations obeyed ยฎrst-order kinetics at the temperature and pH values studied. The optimal