Effect of pressure on solute retention in the ionization control mode in reversed-phase liquid chromatography

✍ Scribed by Nobuo Tanaka; Tadanori Yoshimura; Mikio Araki

• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 654 KB
• Volume: 406
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)94033-0

✦ Synopsis

The pressure in a chromatographic system was found to have a significant effect on the retention of solutes in the ionization control mode in reversed-phase liquid chromatography. An increase in the pressure in a column by 200 kg/cm2 resulted in as much as a 12% variation in k', and caused peak reversal with the variation of the pressure. The variation of retention due to the change in pressure was attributed to the shift of the pH of the mobile phase and a change in the extent of ionization of a solute. The direction and the magnitude of the pressure effect on retention, which depend on the combination of a solute and the type of buffer solution, can be predicted on the basis of the volume changes for ionization of a solute and a buffer species. It was also shown that the effect of pressure on retention can be avoided by the choice of a suitable buffer component in the mobile phase.