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Effect of post-induction substrate oscillation on recombinant alkaline phosphatase production expressed in Escherichia coli

โœ Scribed by Mohammadhadi Jazini; Christoph Herwig


Book ID
113683632
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
576 KB
Volume
112
Category
Article
ISSN
1389-1723

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The effect of post-induction nutrient feeding strategies on the production of bioadhesive protein using an IPTG inducible expression system in Escherichia coli was investigated. Cells were cultured in an exponen- tial fed-batch mode to the OD 600 of ca. 100 (48 gDCW/L) prior to induction. Six differ