Effect of ozone on plasmid DNA of Escherichia coli in situ
โ Scribed by Kozo Ishizaki; Kazuyuki Sawadaishi; Kazunobu Miura; Nariko Shinriki
- Book ID
- 119015046
- Publisher
- IWA Publishing
- Year
- 1987
- Tongue
- English
- Weight
- 510 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0043-1354
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Clorobiocin, an inhibitor of the gyrB subunit of DNA gyrase, was used for the curing of some Escherichia coli plasmids. Of the plasmids studied, ampicillin resistant R28K and a miniplasmid derived from Rldrd-19 were effectively eliminated. We also succeeded in eliminating the ColA factor from E. coi
The objectives of this **Second Edition** of **Biotechnology: A Laboratory Course** remain unchanged: to create a text that consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry techniques while providing a continuum of experiments. The course begins