๐”– Bobbio Scriptorium
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Cotransformation of linear chromosomal DNA and plasmid DNA in Escherichia coli

โœ Scribed by H.E.N. Bergmans; D.H. Kooijman; W.P.M. Hoekstra


Book ID
109311520
Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
282 KB
Volume
9
Category
Article
ISSN
0378-1097

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Monomeric pBR322 DNA that had been linearized at its unique SalI site transformed wild-type Escherichia coli with 102 to 103 times less efficiency than CCC plasmid DNA. Dose-response experiments indicated that a single linear plasmid 'molecule' was sufficient to produce a transformant. Transformatio