## Animprovedvectorof2889bpwM construoted by mutation of copy number control system, into which f~geneswithoutarwiththestartcodanATGcaslbedirectfyinsatedforhigh-levelexpressjonin Ederichiu cd. Deletion of the rop gene mooding a negative regulation protein ROP leads to increase the plssmidcopynumbe
An improved method for detecting foreign DNA in plasmids of Escherichia coli
β Scribed by Ronald E. Thayer
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 1024 KB
- Volume
- 98
- Category
- Article
- ISSN
- 0003-2697
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An improved method for the rapid extraction and purification of the nucleic acids from small amounts of Escherichiu co/i is described. The method is applicable to multiple samples of about 5 X IO" cells each, and can be completed in about 4-6 hr. Recovery is on the order of 60%, there is little dete
## Abstract We set out to investigate if __E. coli__ genotype plays a significant role in host strain selection for optimal processing of plasmid DNA based on both quality and quantity of supercoiling. Firstly 17 __E. coli__ commercial and nonβcommercial strains were selected and their available ge