Plasmid pKKH: An improved vector with higher copy number for expression of foreign genes inEscherichia coli

✍ Scribed by Guozhong Jing; Zhen Huang; Zhige Liu; Qiang Zou

• Publisher: Springer Netherlands
• Year: 1993
• Tongue: English
• Weight: 250 KB
• Volume: 15
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00129314

✦ Synopsis

Animprovedvectorof2889bpwM

construoted by mutation of copy number control system, into which f~geneswithoutarwiththestartcodanATGcaslbedirectfyinsatedforhigh-levelexpressjonin Ederichiu cd. Deletion of the rop gene mooding a negative regulation protein ROP leads to increase the plssmidcopynumber,and8nal&m&esthevectorincmese expmssion level more than 60% in comparison withinitialone.