Overproduction of EcoRI and SpA::EcoRI f
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Xiaoan Wu; Lutz Brandes; Hans E. Maschke; Karl Schügerl
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Article
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2007
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Wiley (John Wiley & Sons)
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English
⚖ 381 KB
The copy numbers of the expression plasmids (pRTF309+ and pMTC48), the repression plasmids (pc1857 and pRK248cI) and the protection plasmid (pEcoR4) in recombinant E. coli JM 103 were investigated. In the absence of the protection plasmid, the copy number of the expression plasmid dropped; in its pr