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Curing effect of clorobiocin on Escherichia coli plasmids

✍ Scribed by Čejka, Karel ;Holubová, Inge ;Hubáček, Josef

Publisher: Springer
Year: 1982
Tongue: English
Weight: 239 KB
Volume: 186
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00422928

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✦ Synopsis

Clorobiocin, an inhibitor of the gyrB subunit of DNA gyrase, was used for the curing of some Escherichia coli plasmids. Of the plasmids studied, ampicillin resistant R28K and a miniplasmid derived from Rldrd-19 were effectively eliminated. We also succeeded in eliminating the ColA factor from E. coil strain B834(pBS103), which was resistant to the effect of currently used curing agents. Although a derivative of ColEl-pBR322 was effectively cured by clorobiocin, the ColEI plasmid was resistant to its effect. The ColV plasmid determining virulence was effectively eliminated.

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