Effect of neonatal blood serum on the early stages of mouse embryogenesisin vitro
β Scribed by I. B. Arkhangel'skaya; V. I. Shtil'bans; N. A. Chebotar'; A. M. Kotin
- Book ID
- 105576863
- Publisher
- Springer US
- Year
- 1994
- Tongue
- English
- Weight
- 335 KB
- Volume
- 118
- Category
- Article
- ISSN
- 0007-4888
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## Abstract Mouse blastocysts cultured in vitro in rat serum develop through the early somitic stages of embryogenesis at the same rate as embryos cultured in human cord serum. Rat serum can thus be used instead of human cord serum for in vitro culturing of mouse embryos.
## Abstract Mouse spleen cells were cultured in the presence or absence of one of the following mitogens: phytohemagglutinin (PHA), concanavalin A, allogeneic spleen cells or bacterial lipopolysaccharide. The addition of normal mouse serum (NMS) to the cultures usually depressed mitogenβinduced lym