Effect of mobile phase additives on resolution of some nucleic compounds in high performance liquid chromatography
β Scribed by Chun Hua Jin; Yoon Mo Koo; Dae-Ki Choi; Kyung Ho Row
- Publisher
- The Korean Society for Biotechnology and Bioengineering
- Year
- 2007
- Tongue
- English
- Weight
- 234 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1226-8372
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π SIMILAR VOLUMES
## Abstract Highly hydrophilic compounds belonging to biogenic amines were analysed in the reversedβphase system, modified with the addition of ionic liquids: 1βethylβ3βmethylβimidazolium hexafluorophosphate (EMIM PF~6~) and chaotropic salt NaPF~6~ on Discovery HS C18 column at acidic conditions. T
Secondary equilibria in reversed-phase liquid chromatography have been investigated as a means of enhancing selectivity and optimizing separations of nucleic acid constituents. The retention behavior of various nucleotides, nucleosides and modified compounds has been examined as a function of live d