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Effect of matrigel on the tumorigenicity of human breast and ovarian carcinoma cell lines

✍ Scribed by Peter Mullen; Alison Ritchie; Simon P. Langdon; William R. Miller

Publisher
John Wiley and Sons
Year
1996
Tongue
French
Weight
534 KB
Volume
67
Category
Article
ISSN
0020-7136

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✦ Synopsis

The effect of Matrigel, a solubilised tissue basement membrane extract, has been investigated on the tumorigenicity of 3 breast (MCF-7, T47D and MDA.MB.23 I) and 5 ovarian [PEOI, PEO I cDDPr, PE04, PEO 14 and OV(hyg)CAR3] carcinoma cell lines. In the absence of Matrigel, the PE014 and MDA.MB.231 cell lines produced take rates of 30% and 50%, respectively, while the other cell lines either did not develop or only occasionally developed as tumours. With Matrigel, 100% take rates were achieved for 7 of the 8 cell lines (MCF-7, T47D, MDA.MB.231, PEOI, PEOI cDDPr, PE04 and PEO14); in the remaining cell line [OV(hyg)CAR3] 2/6 (33"o) tumours grew. Xenografts established with Matrigel could be transferred into recipient animals and grown in the absence of Matrigel, suggesting that Matrigel is necessary only for initial establishment of tumours. Furthermore, cells which had been re-established from a T47D xenograft and then inoculated into mice without Matrigel showed a take rate greater than that of the original cell line but less than that of the xenograft. In conclusion, Matrigel has proven to be extremely useful in establishing a variety of cell lines as xenografts.

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