Effect of insulin on a serum-free hybridoma culture
β Scribed by Weichang Zhou; Wei-Shou Hu
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 436 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0006-3592
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The murine B-lymphocyte hybridoma, CC9C10 was grown at steady state under serum-free conditions in continuous culture at dissolved oxygen (DO) concentrations in the range of 10% to 150% of air saturation. Cells could be maintained within this range at high viability in a steady state at a dilution r
## Abstract The achievement of high cell density and monoclonal antibody concentration depends on understanding cell metabolism and the control of cell culture. A model of cell metabolism and monoclonal antibody production in hybridoma cell culture is presented. Fedβbatch culture in serumβfree medi