Serum-free cell culture on insulin-immobilized porous collagen beads
✍ Scribed by Yoshihiro Ito; Ji Zheng; Yukio Imanishi
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 533 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0006-3592
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The effect of lithium on the growth of mammary epithelial cells from adult virgin and midpregnant BALBlc or BALBlcfC3H mice was tested in a serumfree collagen gel culture system. The serum-free medium consisted of a 1:l mixture of Ham's F12 and Dulbecco's Modified Eagle's medium supplemented with in
## Abstract The mitogenic effect of TGF‐alpha, acidic‐FGF, basic‐FGF and lithium on normal human breast epithelial cells was studied in a collagen gel culture system using a serum‐free 1:1 mixture of Ham's F12 and DME medium containing insulin, cholera toxin and bovine serum albumin. TGF‐alpha elic