✦ LIBER ✦

Effect of growth temperature and media composition on the fatty acid composition ofBacillus stearothermophilusAN 002

✍ Scribed by R. L. Bezbaruah; K. R. Pillai; B. K. Gogoi; J. N. Baruah

Publisher: Springer Netherlands
Year: 1988
Tongue: English
Weight: 435 KB
Volume: 54
Category: Article
ISSN: 0003-6072
DOI: 10.1007/bf00393956

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✦ Synopsis

The influence of growth temperature, media composition and cell age on the chemical composition of Bacillus stearothermophilus strain AN 002 has been determined. The total cellular protein decreased and the free amino acid content increased with growth temperature, in both exponential and stationary growth phase. The protein and free amino acid contents of cells were higher in the stationary phase than in the exponential phase, irrespective of growth temperature and media composition. The RNA content was only reduced in cells grown at 55 degrees C. No significant variations were observed in the DNA and carbohydrate contents with respect to growth temperature and cell age. The total lipid and fatty acid compositions on the other hand varied as a function of growth temperature, cell age and media composition. Differences in the relative concentrations of even, odd and branched chain fatty acids were noticed. No variation was observed in the antiiso and unsaturated fatty acids with respect to growth temperature. The unique variations in the fatty acid composition and total lipids at the growth temperature of 50 degrees C and their variations in the stationary growth phase seem to be characteristic for B. stearothermophilus AN 002.

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