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Effect of growth conditions on the ornithine decarboxylase activity of rat hepatoma cells

✍ Scribed by Brigid L.M. Hogan

Book ID
118852905
Publisher
Elsevier Science
Year
1971
Tongue
English
Weight
349 KB
Volume
45
Category
Article
ISSN
0006-291X

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Effect of growth conditions on the activ
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## Abstract Ornithine decarboxylase activity in high density, stationary phase rat hepatoma (HTC) cells in suspension culture has an extremely short half‐life of between 5 and 15 minutes, as measured after inhibiting protein synthesis. Following dilution of these cells into fresh medium there is a

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Arizona 85724 DH23A cells, an a-difluoromethylornithine (DFMO)-resistant variant of rat hepatoma tissue culture cells (HTC), contain high levels of very stable ornithine decarboxylase (ODC). In the absence of DFMO, the high ODC activity results in a large accumulation of endogenous putrescine. Conco