Effect of α-methyl ornithine on ornithine decarboxylase activity of rat hepatoma cells in culture
✍ Scribed by Peter P. McCann; Chantal Tardif; Marie-Christine Duchesne; Pierre S. Mamont
- Book ID
- 118312095
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 384 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0006-291X
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Arizona 85724 DH23A cells, an a-difluoromethylornithine (DFMO)-resistant variant of rat hepatoma tissue culture cells (HTC), contain high levels of very stable ornithine decarboxylase (ODC). In the absence of DFMO, the high ODC activity results in a large accumulation of endogenous putrescine. Conco
## Abstract Ornithine decarboxylase activity in high density, stationary phase rat hepatoma (HTC) cells in suspension culture has an extremely short half‐life of between 5 and 15 minutes, as measured after inhibiting protein synthesis. Following dilution of these cells into fresh medium there is a