✦ LIBER ✦

Effect of fructose and glucose supplementation on invertase mediated synthesis of oligosaccharides from sucrose

✍ Scribed by Richard Somiari; Stanislaw Bielecki

Publisher: Springer Netherlands
Year: 1995
Tongue: English
Weight: 320 KB
Volume: 17
Category: Article
ISSN: 0141-5492
DOI: 10.1007/bf00132021

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✦ Synopsis

The total amount of novel oligosaccharides synthesized by 13-D-fructofuranosidase at pH 7.5 increased three-fold using a medium composed of 1.2M sucrose, 0.5M fructose and 0.1M glucose, as compared to that with only 1.8M sucrose solution. Using 0.6M of the three sugars did not increase yield but reduced rate of sucrose hydrolysis by 72.7%. Synthesis of fructosyl/glucosyi oligosaccharides based on 13-fructofuranosidase mediated transglycosylation is enhanced by supplementation of sucrose solution with appropriate concentrations fructose and glucose.

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