For the continuous, enzymatic synthesis of sorbitol and gluconic acid by cell-free glucose-fructose oxidoreductase (GFOR) from Zymomonas mobilis, the principal determinants of productivity have been identified. Most important, the rapid inactivation of the soluble enzyme during substrate conversion
Effect of oleic acid on the production of ethanol and fructose from glucose/fructose mixtures in an immobilized cell reactor
✍ Scribed by M. E. Guénette; Z. Duvnjak
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 364 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0138-4988
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✦ Synopsis
Saccharornyces cerevisiae ATCC 39859 was immobilized onto small cubes of wood to produce ethanol and very enriched fructose syrup from glucoselfructose mixtures through the selective fermentation of glucose. A maximum ethanol productivity of 21.9 g 11. h was attained from a feed containing 9.7% (w/v) glucose and 9.9% (w/v) fructose. An ethanol concentration. glucose conversion and fructose yield of 29.6 g / 1, 62% and 99% were obtained, respectively. This resulted in a final fructose / glucose ratio of 2.7. At lower ethanol productivity levels the fructose / glucose ratio increases, as does the ethanol concentration in the effluent. The addition of 30 mg /1 oleic acid to the medium increased the ethanol productivity and its concentration by 13% at a dilution rate of 0.74 h -l.
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