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Effect of Exercise on the Creatine Resonances in1H MR Spectra of Human Skeletal Muscle

✍ Scribed by R. Kreis; B. Jung; J. Slotboom; J. Felblinger; C. Boesch

Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
108 KB
Volume
137
Category
Article
ISSN
1090-7807

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✦ Synopsis

1 H MR spectra of human muscles were recorded before, during, and after fatiguing exercise. In contrast to expectations, it was found that the spectral contributions of creatine/phosphocreatine (Cr/PCr) were subject to change as a function of exercise. In particular, the dipolar-coupled methylene protons of Cr/PCr were found to be reduced in intensity in proportion to the co-registered PCr levels. Recovery after exercise and behavior under ischemic conditions provide further evidence to suggest that the contributions of the CH 2 protons of Cr/PCr to 1 H MR spectra of human muscle in vivo reflect PCr rather than Cr levels. Variation of experimental parameters showed that this effect is not due to a trivial change in relaxation times. At present it can only be speculated about why the Cr resonances have reduced NMR visibility. If temporary binding to macromolecules should be involved, the free Cr concentration-important for equilibrium calculations of the creatine kinase reaction-might be different from what was previously assumed.

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