Effect of continuous heat stress on cell growth and protein synthesis in Aedes albopictus

✍ Scribed by Maria da Gloria da Costa Carvalho; Marcia Soares Freitas

• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 740 KB
• Volume: 137
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041370309

✦ Synopsis

de janeiro, R.]. Brazil Aedes albopictus (clone C6136) cells, which normally grow at 28"C, were maintained at a supraoptimal temperature of 37°C. The effect of continuous heat stress (37°C) on cell growth was analyzed as were the modifications occurring with protein synthesis during short-and long-term heat stress. We observed that cells in lag or exponential growth phase, present inhibition of cell growth, and cells in the lag phase showed more sensitivity to death than cells growing exponentially. During the first hour of exposing the cells to 3 7 T , they synthesized two heat shock proteins (hsps) of 82 kd and 7 0 kd, respectively, concomitant with inhibition of normally produced proteins at control temperature (28°C). However, for incubations longer than 2 hr at 37"C, a shift to the normal pattern of protein synthesis occurred. During these transitions, two other hsps of 76 kd and 90 kd were synthesized. Pulse chase experiments showed that the 70-kd hsp is stable at least for 18 hr, when the cells are returned to 28°C. However, if cells were incubated at 37"C, the 70-kd hsp is stable for at least 48 hr. The 70-kd hsp was localized in the cytoplasmic and in the nuclear compartment. Our results indicate a possible role of hsp 70-kd protein in the regulation of cell proliferation.