To investigate the response of hybridoma cells to hypoosmotic stress, S3H5/โฅ2bA2 and DB9G8 hybridomas were cultivated in the hypoosmolar medium [Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% serum] resulting from sodium chloride subtraction. Both hybridomas showed similar responses
Optimization of tetracycline-responsive recombinant protein production and effect on cell growth and ER stress in mammalian cells
โ Scribed by Jullian Jones; Tarangsri Nivitchanyong; Christina Giblin; Valentina Ciccarone; David Judd; Stephen Gorfien; Sharon S. Krag; Michael J. Betenbaugh
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 321 KB
- Volume
- 91
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Glutamine is a major source of energy, carbon, and nitrogen for mammalian cells. The amount of glutamine present in commercial mammalian cell media is, however, not necessarily balanced with cell requirements. Therefore, the effects of glutamine limitation on the physiology of two mammalian cell lin
The effects of spatial variation of cells and nutrient and product concentration, in combination with product inhibition in cell growth kinetics on chondrocyte generation in a polymer scaffold, are analyzed. Experimental studies reported previously have demonstrated spatial dependence in the cultiva
Background: To gain insight into the role of satellite cells in skeletal muscle hypertrophy, the effect of radiation on small fiber formation, embryonic myosin heavy chain (embryonic MHC) production, and insulin-like growth factor I (IGF-I) production in overloaded adult rat soleus muscle was examin