Effect of temperature and oxygen on cell growth and recombinant protein production in insect cell cultures

✍ Scribed by S. Reuveny; Y. J. Kim; C. W. Kemp; J. Shiloach

• Publisher: Springer
• Year: 1993
• Tongue: English
• Weight: 458 KB
• Volume: 38
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00182800

✦ Synopsis

The effect of temperature and Oz saturation on the production of recombinant proteins fl-galactosidase and human glucocerebrosidase by Spodoptera frugiperda cells (Sf9) infected with recombinant Autographa californica nuclear polyhedrosis virus was investi- gated. The rates of cell growth, glucose consumption, O2 consumption and product expression were measured at temperatures between 22 ° C and 35 ° C. The results indicated that possible O: limitation may be alleviated without compromising the maximum cell yield by lowering the incubation temperature from 27 ° C to 25 ° C. The expression level of the recombinant proteins at 27°C was similar to that obtained at 22°C and 25 ° C; lower protein yields were obtained at 30 ° C. Art increase in temperature from 22°C to 27°C led to earlier production of the proteins and to an increase in the proportion of the product released outside the cells.