Effect of aminoacylation on tRNA conformation
โ Scribed by J. Antosiewicz; D. Porschke
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 256 KB
- Volume
- 17
- Category
- Article
- ISSN
- 1432-1017
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A chromatographic absorbent is described for the column separation of aminoacyl-tRNAs, which combines high resolution with high sensitivity and simplicity. Without using high pressures, 5-1500 pg of tRNA can be fractionated in 60 min. The system is called RPC-LH because it utilizes LH-Sephadex coate
## Abstract Circular dichroism spectra of acridine orange bound to __E. coli__ tRNA were studied at varying tRNA phosphateโtoโdye (P/D) ratios for both unfractionated and purified materials in the absence of Mg^++^. From the rather discrete features exhibited in the circular dichroism spectra three