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E. coli minichromosome replication: regulation of initiation at oriC

✍ Scribed by Crooke, E.; Hwang, D.S.; Skarstad, K.; Thöny, B.; Kornberg, A.


Book ID
120421305
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
200 KB
Volume
142
Category
Article
ISSN
0923-2508

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Overproduction of DnaA protein stimulate
✍ Atlung, Tove ;Løbner-Olesen, Anders ;Hansen, Flemming G. 📂 Article 📅 1987 🏛 Springer 🌐 English ⚖ 1015 KB

Increased synthesis of DnaA protein, obtained with plasmids carrying the dnaA gene controlled by the heat inducible lambda pL promoter, stimulated initiation of replication from oriC about threefold. The overinitiation was determined both as an increase in copy number of a minichromosome and as an i