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Replication initiated at the origin (oriC) of the E. coli chromosome reconstituted with purified enzymes

✍ Scribed by Kaguni, Jon M.; Kornberg, Arthur


Book ID
122517783
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
826 KB
Volume
38
Category
Article
ISSN
0092-8674

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A new round of chromosomal replication of a temperature-sensitive initiation mutant (dnaC) of Escherichia coli was initiated synchronously by a temperature shift from a nonpermissive to a permissive condition in the presence of arabinosyl cytosine. Increased amounts of nascent DNA fragments with hom