DNA replication in Escherichia coli is initiated by membrane detachment of oriC
โ Scribed by Vic Norris
- Book ID
- 119443238
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 735 KB
- Volume
- 215
- Category
- Article
- ISSN
- 0022-2836
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When E. coli F+ cells carrying the dna-167 or dnaC2 mutation, which causes the temperature-sensitive initiation of DNA replication, are exposed to a non-permissive temperature to stop the replication of chromosome and F factor, and then transferred back to a permissive temperature with the addition
Temperature-sensitive mutants defective in the initiation of DNA replication are exposed to a non-permissive temperature to complete already initiated replication, and are transferred back to a permissive temperature. DNA synthesis can resume in the presence of rifampicin or rifampicin plus chloramp