Dynamics of in vivo protein aggregation: building inclusion bodies in recombinant bacteria
✍ Scribed by M.M Carrió; J.L Corchero; A Villaverde
- Book ID
- 117518936
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 308 KB
- Volume
- 169
- Category
- Article
- ISSN
- 0378-1097
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A method is presented for the direct extraction of the recombinant protein Long-R 3 -IGF-I from inclusion bodies located in the cytoplasm of intact Escherichia coli cells. Chemical treatment with 6M urea, 3 mM EDTA, and 20 mM dithiothreitol (DTT) at pH 9.0 proved an effective combination for extract