[Microbiology Monographs] Inclusions in Prokaryotes Volume 1 || Protein Inclusion Bodies in Recombinant Bacteria
β Scribed by Shively, Jessup M.
- Book ID
- 111978470
- Publisher
- Springer-Verlag
- Year
- 2006
- Tongue
- German
- Weight
- 565 KB
- Edition
- 2006
- Category
- Article
- ISBN-13
- 9783540262053
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β¦ Synopsis
Prokaryote Inclusions: Descriptions And Discoveries / J.m. Shively -- Bacterial Sulfur Globules: Occurrence, Structure And Metabolism / C. Dahl, A. Prange -- Acidocalcisomes And Polyphosphate Granules / R. Docampo -- Bacterial Glycogen Inclusions: Enzymology And Regulation Of Synthesis / J. Preiss -- Biogenesis And Structure Of Polyhydroxyalkanoate Granules / M. Potter, A. Steinbuchel -- Wax Ester And Triacylglycerol Inclusions / M. Waltermann, A. Steinbuchel -- Insecticidal Protein Crystals Of Bacillus Thuringiensis / B.a. Federici, H.-w. Park, Y. Sakano -- Protein Inclusion Bodies In Recombinant Bacteria / P. Neubauer, B. Fahnert, H. Lilie, A. Villaverde -- Vacuoles / H.n. Schulz-vogt -- The Unique Role Of Intracellular Calcification In The Genus Achromatium / N.d. Gray -- Metal Inclusions In Bacteria / S. Langley -- Inclusion Protein From Other Insecticidal Bacteria / H.-w. Park, B.a. Federici, Y. Sakano -- R-bodies / A. Sanchez-amat. Volume Editor, Jessup M. Shively. Includes Bibliographical References And Index. Mode Of Access: World Wide Web.
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A method is presented for the direct extraction of the recombinant protein Long-R 3 -IGF-I from inclusion bodies located in the cytoplasm of intact Escherichia coli cells. Chemical treatment with 6M urea, 3 mM EDTA, and 20 mM dithiothreitol (DTT) at pH 9.0 proved an effective combination for extract