Protein aggregation in recombinant bacteria: biological role of inclusion bodies
✍ Scribed by Antonio Villaverde; M. Mar Carrió
- Book ID
- 111547178
- Publisher
- Springer Netherlands
- Year
- 2003
- Tongue
- English
- Weight
- 149 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0141-5492
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A method is presented for the direct extraction of the recombinant protein Long-R 3 -IGF-I from inclusion bodies located in the cytoplasm of intact Escherichia coli cells. Chemical treatment with 6M urea, 3 mM EDTA, and 20 mM dithiothreitol (DTT) at pH 9.0 proved an effective combination for extract
In previous parts of this study we developed procedures for the high-efficiency chemical extraction of soluble and insoluble protein from intact Escherichia coli cells. Although high yields were obtained, extraction of recombinant protein directly from cytoplasmic inclusion bodies led to low product