✦ LIBER ✦

Dynamic aspects of rhodamine dye photosensitization in vitro with an argon-ion Laser

✍ Scribed by Dr. Christopher R. Shea; Norah Chen; Tayyaba Hasan

Publisher: John Wiley and Sons
Year: 1989
Tongue: English
Weight: 615 KB
Volume: 9
Category: Article
ISSN: 0196-8092
DOI: 10.1002/lsm.1900090202

No coin nor oath required. For personal study only.

✦ Synopsis

Phototoxicity in cultured human bladder carcinoma cells treated by 514.5-nm argon-ion laser irradiation plus rhodamine-123 (R123) or tetrabromo-R123 (TBR) was assessed by counting total cell number and percent viability at 1, 24, 48, 72, and 96 h after irradiation. TBR was markedly more efficient than R123 at causing both reversible and persistent phototoxic cytostasis. Furthermore, TBR photosensitization caused net cytocidal effects at 0.5 J/cm2, which were not seen with R123 photosensitization at up to 40 J/cm2. The reduced phototoxic efficiency of R123 as compared to TBR appears, in part, to reflect the presence of a fraction of cells refractory to R123 photosensitization.

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