Direct comparison of in-vitro and in-vivo photofrin-II mediated photosensitization using a pulsed KTP pumped dye laser and a continuous wave argon ion pumped dye laser
✍ Scribed by Angela Ferrario; Natalie Rucker; Stefan W. Ryter; Daniel R. Doiron; Dr. Charles J. Gomer
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 574 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0196-8092
No coin nor oath required. For personal study only.
✦ Synopsis
A pulsed KTP pumped dye laser (25 kHz repetition rate and 470 nsec pulse width) has been compared to a continuous wave argon ion pumped dye laser as the source of 630 nm light during in-vitro and in-vivo Photofrin-I1 mediated photosensitization studies. Individual experiments documented the effectiveness of each laser system on a) photosensitizer induced cytotoxicity and induction of stress protein synthesis using Chinese hamster fibroblasts; b) photobleaching of Photofrin-I1 in aqueous solution; c) Photofrin I1 mediated photosensitization of normal mouse skin; d) Photofrin I1 mediated photodynamic therapy of a mouse mammary carcinoma; and e) tumor temperature levels generated during laser exposure. Comparable results were obtained for both laser systems in all experiments.