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Doubled haploid production in Flax (Linum usitatissimum L.)

✍ Scribed by Bohuš Obert; Zuzana Žáčková; Jozef Šamaj; Anna Preťová


Book ID
113494705
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
166 KB
Volume
27
Category
Article
ISSN
0734-9750

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A method for isolation of flax RNA is described; properties of the isolated RNA are given. RNA degradation was held to a minimum through a high pH (9.5) extraction buffer, diethylpyrocarbonate (4%) as a nuclease inhibitor, a high concentration (1.5%) of sodium dodecylsulphate, 2 mM Mg", and separati