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Isolation and characterisation of RNA from flax (Linum usitatissimum L.)

✍ Scribed by M.Raafat El-Gewely; Hugh Tyson


Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
729 KB
Volume
62
Category
Article
ISSN
0003-2697

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✦ Synopsis


A method for isolation of flax RNA is described; properties of the isolated RNA are given. RNA degradation was held to a minimum through a high pH (9.5) extraction buffer, diethylpyrocarbonate (4%) as a nuclease inhibitor, a high concentration (1.5%) of sodium dodecylsulphate, 2 mM Mg", and separation of the RNA from contaminating materials on Sephadex G-50.


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