RFLP and RAPD mapping in flax (Linum usitatissimum)
โ Scribed by T. J. Oh; M. Gorman; C. A. Cullis
- Publisher
- Springer
- Year
- 2000
- Tongue
- English
- Weight
- 65 KB
- Volume
- 101
- Category
- Article
- ISSN
- 0040-5752
No coin nor oath required. For personal study only.
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A method for isolation of flax RNA is described; properties of the isolated RNA are given. RNA degradation was held to a minimum through a high pH (9.5) extraction buffer, diethylpyrocarbonate (4%) as a nuclease inhibitor, a high concentration (1.5%) of sodium dodecylsulphate, 2 mM Mg", and separati
## ABsTRAcr The EDTA-extracted pectins from flax, which were fractionated by exclusion chromatography according to size, contained galactose, galacturonic acid, rhamnose , glucose, and traces of arabinose. The main components of the major fraction, a high-molecular-weight galactan, were +4)-p-Galp