✍ Scribed by Ernest L. Wynder; Jürgen W. Spranger; Martin M. Fark
No coin nor oath required. For personal study only.
T IS A well established principle that tumor I formation is influenced by the concentration, duration of application, and the amount in which a carcinogen is applied. Studies with tobacco smoke condensate showed that tumor formation was dependent upon the total amount of condensate received rather than on the painting schedule itself.' These data also showed that when the condensate was applied for 12 months, any additional application did not seem to increase tumor yield. The present experiment was designed to duplicate the experimental conditions of the dose-response study with tobacco smoke condensate, using a single carcinogen in low concentration.
The experiment was conducted with Swiss mice obtained from Millerton Farms, Miller-ton, N.Y. Each experimental group consisted of 20 mice. All of the animals received benzo-[ulpyrene in a 0.00570 acetone solution. This solution was applied to the backs of the animals with a no. 5 camel's-hair brush. The solution was freshly prepared every 2 weeks and stored in clear glass bottles in a refrigerator at 7" C. The backs of all animals were shaved whenever hair growth recurred.
The 10 series fall into 3 main classes. In a dose-response series consisting of groups 1, 2, 3, and 4 the solution was applied 1, 2, 3, and 5 times a week for the life span of the animal. An interval series consisted of groups 5 and 6. The animals were painted 3 times a week for 12 months. Comparable doses were applied to the 2 groups; group 5, however, was painted on an alternate painting and nonpainting schedule of 2 weeks and group 6 on an alternate painting and nonpainting schedule of 4 weeks. They are compared to group 8, which received the same hose but benzopyrene solution was applied 3 times a 12 months, as well as for the entire life span (groups 7, 8, 9, 10, and 3). From the Section of Epidemiology. Division of Preventive Medicine, Sloan-Kettenng Institute for Cancer was painted continuously. In a third series the Research, New York, N.Y.
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