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DNase I sensitivity of ribosomal genes in isolated nucleosome core particles

✍ Scribed by Giri, Chandrakant P.; Gorovsky, Martin A.


Book ID
121671808
Publisher
Oxford University Press
Year
1980
Tongue
English
Weight
703 KB
Volume
8
Category
Article
ISSN
0305-1048

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## Abstract HeLa cells were synchronized at late G1, early S, and late S phase of the cell cycle by nocodazole treatment. The cells were permeabilized with Triton X‐100, digested with DNAse I, and extracted with 0.2 M ammonium sulfate to remove the digested chromatin. DNA was isolated from the resi