DNase I sensitive site in the core region of the human β-globin origin of replication

✍ Scribed by Vera Djeliova; George Russev; Boyka Anachkova

• Book ID: 102300152
• Publisher: John Wiley and Sons
• Year: 2002
• Tongue: English
• Weight: 86 KB
• Volume: 87
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.10298

✦ Synopsis

Abstract

HeLa cells were synchronized at late G1, early S, and late S phase of the cell cycle by nocodazole treatment. The cells were permeabilized with Triton X‐100, digested with DNAse I, and extracted with 0.2 M ammonium sulfate to remove the digested chromatin. DNA was isolated from the residual chromatin attached to the nuclear matrix, digested with Hind III, and subjected to hybridization with [^32^P] labeled probe located upstream of the core region of the human β‐globin replication origin. The hybridization pattern revealed the existence of a DNase I sensitive site in the core region of the β‐globin replicator. The results suggest that association with the nuclear matrix induce alteration in the chromatin structure of the origin of replication that represents a more open chromatin configuration. J. Cell. Biochem. 87: 279–283, 2002. © 2002 Wiley‐Liss, Inc.