DNA Synthesis in vitro
β Scribed by SMITH, DOUGLAS W.; SCHALLER, HEINZ E.; BONHOEFFER, FRIEDRICH J.
- Book ID
- 109673501
- Publisher
- Nature Publishing Group
- Year
- 1970
- Tongue
- English
- Weight
- 320 KB
- Volume
- 226
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/226711a0
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π SIMILAR VOLUMES
A system is described in which DNA synthesis can be monitored in a yeast lysate. The observed synthesis has many of the properties of in vivo DNA replication. It is dependent upon replication growing points that were active in vivo. The in vitro synthesis proceeds via low molecular weight intermedia
Both the polymerase and the exonuclease activities of DNA polymerase III are inactivated by treatment with nitrosoguanidine. The treatment of the DNA template with the mutagen does not affect the template in supporting DNA synthesis. No effect of nitrosoguanidine upon fidelity of replication in vitr